The Extraction and Purification of DNA.
Deoxyribonucleic Acid, commonly referred to as DNA, is the molecule found in everything termed as living. The molecule contains genetic code of every organism thus a blueprint which acts as continuous plans for any substance that these organisms do produce at the course of their living. As much as the molecule has a popular usage, very few individuals have actually seen this molecule; actually the molecule happens to be too small for one to see. However, one can carry out easy experiments right at home or in school using some household materials to extract DNA molecules even from plants like peas.
Some of the essential materials you will need for the extraction process include alcohol, meat tenderizer, split peas, dishwashing detergent probably in liquid form, a blender, a beaker or clear glass container, a stirring rod or a spoon. To begin with, blend at least a half cup of split peas in a cupful of water. With that, you should obtain a soupy mixture then use a homogenous solution between the mixtures. One may also consider keeping the extra solution just in case. Then, pour some of the solutions you have, an adequate amount of it into a container up to a level of at least two inches.
In the third step, you need to add at least a tablespoonful of the liquid dishwashing detergent on top of the solution. Again stir the mixture slowly for about five minutes. Next consider adding a pinch of the meat tenderizer the stir again for five minutes. Having stirred the mixture, and then drizzle some alcohol above it. One should consider drizzling the alcohol until they obtain a layer of three inches on the split peas solution. Peas are particularly considered during the extraction because of their high source of this molecule. The use of the detergent is to break down the membrane of the cell hence making it release the contents and the meat tenderizer to dissolve the membrane of the nuclear in order to make it release the DNA inside. The alcohol is used to bring out the DNA strands.
During the purification process, there are a variety of processing kits that can be used. This simply is attributed by the fact that different samples work with different kind of tissues, forensic samples, plant parts and also different cellular structures. Continuously, there are new inventions being made on machines and cutting edge products through different forms of amplification and facilitations for use in new projects and experiments. One such way involves use of magnetized beads which happens to be very effective. Another effective way of purification involves the use of plasmid purification kits that enable the production of low endotoxin levels for use in the subject transfection.